Molecular characterization of exocortis (Citrus exocortis viroid, CEVd) in the Gharb region
Authors
M'Bark OUANTAR, Imane BIBI, Bouchra CHEBLI, Abdelmoughit AIT FRIHA, Mohamed AFECHTAL
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Journal
Published in Vol. 6 No. 1 (2018) of Moroccan Journal of Agricultural and Veterinary Sciences.
Multidisciplinary journal that publishes original research articles and literature reviews in the fields of agriculture, agronomy, rural engineering, topography, forestry, horticulture, food, nutrition, animal husbandry and veterinary science (online-ISSN: 2550-4401, print-ISSN: 2028-991X). Indexed by DOAJ, Google Scholar, Articles DOAJ, Mirabelle, ROAD, WorldCat
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Abstract
Viroids are graft and mechanically transmissible agents, disseminated through budding. A field survey was conducted, during July 2013, for citrus viroids in the Gharb region. A total of 53 samples were collected and used for inoculation of 'Etrog' lemon before being tested by RT-PCR for the detection of exocotris (Citrus exocortis viroid, CEVd). The results showed that CEVd was widespread in the region with an infection rate of 84.9% in the analyzed samples. After genomic sequencing, a study of the genetic diversity was conducted on three representative local isolates of CEVd. The comparison between the nucleotide sequences of Moroccan CEVd isolates revealed a genetic diversity of less than 3 %.
Keywords: Citrus, Citrus exocortis viroid, RT-PCR, sequencing, Gharb.
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Revue Marocaine des Sciences Agronomiques et Vétérinaires est mis à disposition selon les termes de la licence Creative Commons Attribution - Pas d’Utilisation Commerciale - Partage dans les Mêmes Conditions 4.0 International.
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